| 质粒名称: | pCAG-HA-Nrxn1beta AS4(-) |
|---|---|
| 出品公司： | adggene |
| 目录编号: | 59409 |
| 存储实验室: | Peter Scheiffele |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG |
| 空载体大小: | 4869 bp |
| 完整质粒大小: | 6213bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Nrxn1beta AS4(-) / Neurexin 1beta AS4(-) /Neurexin 1beta Delta4 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1344 bp |
| 启动子: | CAG |
| 标签/融合蛋白: | HA (N terminal on insert) |
| 克隆方法: | 未知 |
| 5’测序引物 | pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3') |
| 3’测序引物 | Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3') |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-HA-Nrxn1beta AS4(-) was a gift from Peter Scheiffele (Addgene plasmid # 59409 ; http://n2t.net/addgene:59409 ; RRID:Addgene_59409) |
| 参考文献部分: | Alternative splicing controls selective trans-synaptic interactions of the neuroligin-neurexin complex. Chih B, Gollan L, Scheiffele P. Neuron. 2006 Jul 20. 51(2):171-8. 10.1016/j.neuron.2006.06.005 PubMed 16846852 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pCAG-HA-Nrxn1beta AS4(-) 6213 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-)
[lacking the insert at alternatively spliced segment 4 (AS4-)] .
ACCESSION .
VERSION .
KEYWORDS pCAG-HA-Nrxn1beta AS4(-)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6213)
AUTHORS Chih B, Gollan L, Scheiffele P
TITLE Alternative splicing controls selective trans-synaptic interactions
of the neuroligin-neurexin complex.
JOURNAL Neuron. 2006 Jul 20. 51(2):171-8.
PUBMED 16846852
REFERENCE 2 (bases 1 to 6213)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6213
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 368..645
/label=chicken beta-actin promoter
intron 647..1664
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1588..1610
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1672..1691
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1909..1935
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(3217..3236)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3282..3337
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3336..3355)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3698..3714)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3698..3714)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3711..3733)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3722..3738
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3746..3776)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3791..3812
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3871..4066
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3917..4052
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3979..3998
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4072..4206
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4109..4128)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4163..4182
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4274..4291)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4445..5033)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4525..4544)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5204..6064)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5827..5846
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6065..6169)
/gene="bla"
/label=AmpR promoter
enhancer 6200..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc gctaccggac
1741 tcagatctcg agctcaagct tgggaccatg gcacttccca gatgcatgtg gccaaattat
1801 gtttggagag ctatgatggc atgtgtggtc cacaggggat ccggtgcccc attgactctc
1861 tgcttgttgg gatgtttgct acagactttt cacgtactct ctcaaaagta tccatacgac
1921 gttccggact acgcaggtac cgcatccagt ttgggagcgc accacatcca ccatttccat
1981 ggcagcagca agcatcattc agtgcctatt gcaatctaca ggtcaccagc atccttgcga
2041 ggcggacacg ctgggacaac atatatcttt agcaaaggtg gtggacagat tacatataag
2101 tggcctccca atgaccgccc cagtacacga gcagacaggc tggccatcgg atttagcact
2161 gtccagaagg aagccgtgtt ggtgcgtgtg gacagttcct caggcctggg tgactacctg
2221 gagctgcaca tacaccaagg aaaaattgga gttaagttta atgttgggac agatgacatc
2281 gccatcgaag agtctaatgc aatcattaat gatgggaaat accatgtagt acgtttcaca
2341 aggagtggtg gcaatgccac gttacaggtg gacagctggc cagttatcga acgctaccct
2401 gcagggcgtc agctcacaat cttcaatagc caagcaacca taataattgg cgggaaagag
2461 cagggccagc ccttccaggg ccagctctct ggtctttact acaatggctt gaaagttctg
2521 aatatggcag cagagaacga tgccaacatc gccatagtgg ggaatgtgag gctggtcggt
2581 gaagtgcctt cctctatgac aactgagtcg acagccactg ccatgcagtc tgagatgtcc
2641 acctcaatca tggagaccac cacaaccctg gctaccagca cagctagaag aggcaagccc
2701 cccacaaagg aacccatcag ccagaccaca gatgacatcc ttgtggcctc ggcagagtgt
2761 cccagtgacg atgaggacat tgacccctgt gagccgagct cagccaaccc caccagagta
2821 ggtggccgtg aaccataccc aggctctgca gaggtgattc gggagtccag cagtaccact
2881 ggcatggtgg tggggattgt cgcggcagcc gctctgtgca tcctcatcct cctctatgcc
2941 atgtacaagt acaggaaccg ggatgaaggg tcgtaccacg tggatgagag tcgaaactac
3001 atcagtaact cagcacagtc caatggggct gtggtcaagg agatgcaacc cagcagtgct
3061 aaaagcgcca acaaaaacaa gaagaacaag gataaggagt attatgtctg actcgaggta
3121 cccccgggga gctcaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
3181 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
3241 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
3301 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
3361 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
3421 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
3481 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
3541 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
3601 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
3661 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
3721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
3781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
3841 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
3901 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
3961 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
4021 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
4081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
4141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
4201 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
4261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
4321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
4381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
4441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
4501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
5041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
5101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
5161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
5221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
5281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
5341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
5401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
5461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
5521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
5581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
5641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
5701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
5761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
5821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
5881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
5941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
6001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
6061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
6121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
6181 gaaaagtgcc acctgggtcg acattgatta ttg
//
